EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.9 | additional information | creation of 4-hydroxyphenylacetate-negative mutants by minimal salt medium with ethylmethanesulfonate | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | Escherichia coli | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | Escherichia coli B / ATCC 11303 | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | Escherichia coli C | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
1.13.11.15 | 3,4-dihydroxyphenylacetate + O2 | Escherichia coli | catabolism of 3-hydroxyphenylacetate and 4-hydroxyphenylacetate by 3,4-dihydroxyphenylacetate pathway | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | Escherichia coli | degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | Escherichia coli B / ATCC 11303 | degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | Escherichia coli C | degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.60 | Escherichia coli | - |
- |
- |
1.2.1.60 | Escherichia coli B / ATCC 11303 | - |
- |
- |
1.2.1.60 | Escherichia coli C | - |
- |
- |
1.13.11.15 | Escherichia coli | - |
- |
- |
1.14.14.9 | Escherichia coli | - |
- |
- |
1.14.14.9 | Escherichia coli C | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.1.60 | cell culture | - |
Escherichia coli | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.14.9 | 0.026 | - |
with 3-hydroxyphenylacetate as substrate | Escherichia coli |
1.14.14.9 | 0.028 | - |
with 4-hydroxyphenylacetate as substrate | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | Escherichia coli | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | Escherichia coli B / ATCC 11303 | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | Escherichia coli C | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+ | 1,5% of the activity compared to reduction with NAD+ | Escherichia coli | 5-carboxymethyl-2-hydroxymuconate + NADPH | - |
? | |
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+ | 1,5% of the activity compared to reduction with NAD+ | Escherichia coli B / ATCC 11303 | 5-carboxymethyl-2-hydroxymuconate + NADPH | - |
? | |
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+ | 1,5% of the activity compared to reduction with NAD+ | Escherichia coli C | 5-carboxymethyl-2-hydroxymuconate + NADPH | - |
? | |
1.13.11.15 | 3,4-dihydroxyphenylacetate + O2 | - |
Escherichia coli | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
1.13.11.15 | 3,4-dihydroxyphenylacetate + O2 | catabolism of 3-hydroxyphenylacetate and 4-hydroxyphenylacetate by 3,4-dihydroxyphenylacetate pathway | Escherichia coli | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis | Escherichia coli | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
ir | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway | Escherichia coli | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis | Escherichia coli B / ATCC 11303 | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
ir | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway | Escherichia coli B / ATCC 11303 | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis | Escherichia coli C | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
ir | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway | Escherichia coli C | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
1.14.14.9 | additional information | 3- and 4-hydroxyphenylacetate are both catalyzed by 3- or 4-hydroxyphenylacetate hydroxylase by the same route with 3,4-dihydroxyphenylacetate as the first common intermediate, cells grown on one compound are fully induced for the catabolism of the other | Escherichia coli | ? | - |
? | |
1.14.14.9 | additional information | 3- and 4-hydroxyphenylacetate are both catalyzed by 3- or 4-hydroxyphenylacetate hydroxylase by the same route with 3,4-dihydroxyphenylacetate as the first common intermediate, cells grown on one compound are fully induced for the catabolism of the other | Escherichia coli B / ATCC 11303 | ? | - |
? | |
1.14.14.9 | additional information | 3- and 4-hydroxyphenylacetate are both catalyzed by 3- or 4-hydroxyphenylacetate hydroxylase by the same route with 3,4-dihydroxyphenylacetate as the first common intermediate, cells grown on one compound are fully induced for the catabolism of the other | Escherichia coli C | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.9 | 22 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.9 | 7.8 | - |
assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.60 | NAD+ | - |
Escherichia coli | |
1.2.1.60 | NADP+ | 98.5% lower activity compared to NAD+ | Escherichia coli | |
1.14.14.9 | NADH | can not be replaced by NADPH | Escherichia coli |